The polynucleotide of claim 94, wherein the H4 domain of said mini-dystrophin protein comprises amino acid numbers 3041-3112 from SEQ ID NO:25.ġ04. The polynucleotide of claim 94, wherein the R24 domain of said mini-dystrophin protein comprises amino acid numbers 2932-3040 from SEQ ID NO:25.,ġ03. The polynucleotide of claim 94, wherein the R23 domain of said mini-dystrophin protein comprises amino acid numbers 2803-2931 from SEQ ID NO:25.ġ02. The polynucleotide of claim 94, wherein the R22 domain of said mini-dystrophin protein comprises amino acid numbers 2687-2802 from SEQ ID NO:25.ġ01. The polynucleotide of claim 94, wherein the H3 domain of said mini-dystrophin protein comprises amino acid numbers 2424-2470 from SEQ ID NO:25.ġ00. The polynucleotide of claim 94, wherein the R2 domain of said mini-dystrophin protein comprises amino acid numbers 448-556 from SEQ ID NO:25.ĩ9. The polynucleotide of claim 94, wherein the R1 domain of said mini-dystrophin protein comprises amino acid numbers 337-447 from SEQ ID NO:25.ĩ8. The polynucleotide of claim 94, wherein the 1 domain of said mini-dystrophin protein comprises amino acid numbers 253-327 from SEQ ID NO:25.ĩ7. The polynucleotide of claim 94, wherein the N-terminal Actin Binding Domain of said mini-dystrophin protein comprises amino acid numbers 1-240 from SEQ ID NO:25.ĩ6.
A polynucleotide comprising a nucleic acid sequence encoding a human mini-dystrophin protein, wherein said mini-dystrophin protein comprises the following domains from full-length wild type human muscle dystrophin protein in order from amino-terminus to carboxy-terminus: N-terminal Actin-Binding Domain, hinge H1, rod R1, rod R2, hinge H3, rod R22, rod R23, rod R24, hinge H4, Cysteine-Rich Domain, and the Carboxy-Terminal Domain not including the last 3 amino acids in wild type human muscle dystrophin.ĩ5.
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